FIGURE 1.

Biphasic regulation of HMGR in skin repair. a, regulation of HMGR mRNA expression in skin wounds as assessed by RNase protection assay. The time after injury is indicated at the top of each lane. Ctrl skin refers to back skin biopsies of non-wounded mice. 1000 cpm of the hybridization probe were used as site marker. Hybridization against GAPDH was used as a loading control. A quantification of HMGR mRNA (PhosphorImager PSL counts per 15 μg of total wound RNA) is shown in the lower panel.^*, p < 0.05 (ANOVA, Dunnett's method) compared with control skin. Bars indicate the mean ± S.D. obtained from wounds (n = 48) isolated from animals (n = 12) from three independent animal experiments. b, HMGR activity assays of wound tissue assessed using 3-methyl [3-¹⁴C]glutaryl coenzyme A as substrate. ^*, p < 0.05 (ANOVA, Dunnett's method) compared with control skin. Bars indicate the mean ± S.D. obtained from wounds (n = 24) isolated from animals (n = 12) from three independent animal experiments.