FIGURE 5.

Unique structural features of the CD of EcMazG. A, amino acid sequences composing the fourth and fifth α-helices of MazG-like proteins were aligned. An additional region is identified by a green box. The extended region of α9 in the CD of EcMazG is indicated by a red box and circle. α5 and α10 from the ND and the CD of EcMazG, respectively, are indicated by a blue box and circle. B, hydrophobic interactions to form a rigid additional region of the CD. An extended region of the α9 is indicated, and five nonpolar residues involved in the hydrophobic interactions between α9 and α10 are presented with a gray stick model. C, EcMazG CD was superimposed with SsMazG and CvMazG, both of which lack additional region and cover only the rigid core region of the EcMazG CD. The right image is 90° vertically rotated from the left image.