FIGURE 1.

aak-1 and aak-2 affect paraquat sensitivity of adult C. elegans worms. A, a schematic representation of the aak-2 gene (T01C8.1a in the WormBase Database) in wild-type and mutant strains. B, survival of paraquat-treated wild-type N2 and aak-2 mutant C. elegans worms. Young adult C. elegans worms were incubated in 100 mm paraquat solution, and survival was scored under a stereomicroscope at 5-h intervals. Fifty worms of each strain were treated with paraquat, and the experiment was performed three times. p > 0.05 at 10- and 15-h time points between any pair of aak-2 alleles, except for rr48 at the 10-h time point, where the p value was <0.003 compared with the two other alleles. C, paraquat sensitivities of single and double deficient strains for aak-2 and aak-1. p > 0.15 between N2 and aak-1 at 10 and 15 h, and p < 0.03 between aak-1(tm1944); aak-2(gt33) and aak-2(gt33) at 10 and 15 h. Error bars are S.E.