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A, schematic illustrations of the recognition sites and cleavage styles of Type IIP, Type IIE, and Type IIF restriction endonucleases. B, proposed binding and cleavage mechanism for EcoRII, one of the Type IIE restriction enzymes. EcoRII is a homodimer with an N-domain showing specific binding and a C-domain with catalytic cleavage capacity that was originally covered by the N-domain.
