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. 2008 May 30;283(22):15201–15208. doi: 10.1074/jbc.M800708200

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Copyright © 2008, The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 2. — Decreased expression of cell cycle regulators in FHL2^-/- cells. A, immortalized WT clone B and FHL2^-/- clone b were synchronized by serum starvation and stimulated by 10% serum. Cell lysates were prepared at the indicated time after stimulation and analyzed by immunoblotting. Similar results were obtained with the other two independent clones in each genotype. B, analysis of the expression of Rb and p16^INK4a in seven immortalized clones for each genotype by immunoblotting. The asterisks in A and B show hyperphosphorylated Rb. C, lysates from WT and FHL2^-/- cells were analyzed by immunoblotting with antiphosphospecific antibodies that recognize pRb phosphorylated at specific amino acid residues. D, expression of CDKs was not altered in FHL2^-/- cells. Lysates from WT and FHL2^-/- cells were analyzed by immunoblotting. E, reduced expression of Cdk inhibitors in FHL2^-/- cells. Cells lysates were prepared from WT and FHL2^-/- cells and analyzed by immunoblotting with the indicated antibodies.