FIGURE 7.
C terminus of Lck functions as a lipid raft targeting sequence. 106 3T3-FynT cells infected with Migr1 vector containing either WT Lck (WT), Y505F Lck (Y505F), Y505F+Y394F Lck (Y394F505F), Y505F+K273R Lck (K273R505F), Y505F+ΔQP Lck (ΔQP505F), Y505F+ΔQPQP Lck (ΔQPQP505F), or ΔFQPQP Lck cDNA were lysed in TKM buffer +0.5% Brij-58 and subjected to sucrose gradient centrifugation. 10 μl of each fraction was probed with cholera toxin B subunit-HRP detecting lipid raft marker GM1 (left panels), anti-Fyn (middle panels), and anti-Lck (right panels). Numbers below the panels indicate the percentage distribution of Lck and Fyn to lipid rafts (R) and membrane-soluble (S) fractions in each variant.