Construction and expression of the fusion protein of LIF-Fc.
A, plasmid vector to produce LIF-Fc was constructed as described
under “Experimental Procedures.” B, expression of fusion
protein was checked by Western blotting. IB, immunoblot; Ab,
antibody. The fusion protein was detected by either an anti-mouse IgG antibody
or anti-LIF antibody. C, LIF-Fc was separated by SDS-PAGE under a
reduced or native condition.