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Construction and expression of the fusion protein of LIF-Fc. A, plasmid vector to produce LIF-Fc was constructed as described under “Experimental Procedures.” B, expression of fusion protein was checked by Western blotting. IB, immunoblot; Ab, antibody. The fusion protein was detected by either an anti-mouse IgG antibody or anti-LIF antibody. C, LIF-Fc was separated by SDS-PAGE under a reduced or native condition.
