TABLE 1.
PCR primers used in this study
| Gene (Unigene symbol) | 5′ primer (5′ to 3′) | 3′ primer (5′ to 3′) | Product length | Annealing temperature | Cycle no. |
|---|---|---|---|---|---|
| bp | °C | ||||
| Oct-3/4 (Mm.17031) | GAAGTTGGAAAGGTGGAACC | GCCTCATACCTTCTCCGTTGG | 528 | 60 | 20 |
| TCTTTCCACAGGCCCCCGGCTC | TGCGGGCGGCATGGGGAGATCC | 224 | 60 | 20 | |
| Zfp42/Rex-1 (Mm.285848) | AAAGTGAGATAGCCCCGAG | TCCCATCCCTTCAATAGCA | 930 | 60 | 20 |
| ACGAGTGGCGTTTCTTCTTGGGA | TATGACTCATTCCAGGGGGCACT | 287 | 60 | 20 | |
| Nanog (Mm.6047) | GAGGAAGCACGAATTCTGG | AAGTTATGGGCGGAGCAGC | 710 | 60 | 20 |
| GCGGCTCACTCCTTCTGACTT | GACCAGGAAACCCACACTCAT | 163 | 60 | 20 | |
| Wnt3 (Mm.159091) | TAGAGCTAGCTCCGGGCGATGA | TTGCCTTAACAAGACCACGAAA | 297 | 62 | 20 |
| Actcl (Mm.686) | CCAGATCATTTTGAGACCTTCAA | GAACATTATAGTTACACCATCGC | 124 | 59 | 30 |
| Nkx2.5 (Mm.41974) | GAGCCTACGTGACCCTGACCCAG | TGACCTGCGGGACGTGAGCTTCA | 264 | 60 | 30 |
| Gata4 (Mm.247669) | CTGGAGGCGGATGGGACGGGACACTAC | CCGCAGGCATACATACAGGCTCACC | 207 | 62 | 24 |
| α-Fetoprotein (Mm.358570) | TCGTATTCCACAGGAGG | AGGCTTTTGTTCACCAG | 173 | 55 | 30 |
| Bmp2 (Mm.103205) | GGGACCCGCGTCTTCTAGTGTTGC | TGAGTGCCTCGGTACAGATCTAGCA | 249 | 60 | 30 |
| Polr2a (Mm.16533) | CTGATGCGGTGCTGAGTGAGAAGG | GCGGTTGACCCATGACGAGTG | 237 | 60 | 24 |
| Gapdh (Mm.333399) | CTCATGACCCAGTCCATGC | CTCTTGCTCGTGTCCTTGC | 532 | 60 | 20 |