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CD81 binding. The envelope proteins were either mock treated (0Δ) or mercaptoethanol-treated to cleave 1–9 disulfides/molecule (1–9Δ) prior to iodoacetamide treatment to prevent reoxidation. CD81 binding was assessed by ELISA using CD81-coated wells. Envelope binding was detected using an anti-Fc peroxidase-coupled system (n = 2; duplicates were performed; means of one experiment are shown).
