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Reactivity with mAbs. The envelope proteins were either mock treated (0Δ) or mercaptoethanol-treated to cleave 1–9 disulfides/molecule (1–9Δ), treated using iodoacetamide, and then trapped via protein G on plastic wells. mAb binding (1:300) was detected using an anti-Fab′ peroxidase-coupled system (n = 2; duplicates were performed; means of one experiment are shown).
