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TLC pattern of purified products from WsaC and WsaF activity assays using octyl-linked oligosaccharides. Products were separated on silica TLC plates with chloroform/methanol/water (65:25:4) as solvent. Lane 1, substrate (II) α-l-Rha-(1→3)-β-d-Gal-(1→O)-octyl; lane 2, product of WsaF (IV); lane 3, substrate (III)α-l-Rha-(1→3)-α-l-Rha-(1→3)-β-d-Gal-(1→O)-octyl; lane 4, products of WsaC (V and VI). Staining was performed with thymol reagent.
