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. 2008 Jul 25;283(30):21120–21133. doi: 10.1074/jbc.M801833200

FIGURE 6.

FIGURE 6.

Autoradiogram of methyltransferase activity assays of WsaE and the different truncated versions of WsaE, using α-l-Rha-(1→3)-α-l-Rha-(1→3)-β-d-Gal-(1→O)-octyl (III) (lanes 1-4, 11, and 12), α-l-Rha-(1→3)-β-d-Gal-(1→O)-octyl (II) (lanes 5 and 6), the reaction products of WsaC α-l-Rha-(1→3)-α-l-Rha-(1→3)-α-l-Rha-(1→3)-β-d-Gal-(1→O)-octyl (V and VI) (lanes 7 and 8), α-l-Rha-(1→2)-α-l-Rha-(1→3)-α-l-Rha-(1→3)-β-d-Gal-(1→O)-octyl (VII) (lanes 9 and 10), or α-l-Rha-(1→3)-β-l-Rha-(1→2)-α-l-Rha-(1→3)-β-d-Gal-(1→O)-octyl (IX) (lanes 13 and 14) as substrate. In lanes 1, 6, 8, 10, 12, and 14, dTDP-β-l-Rha was added; all lanes except for lanes 3 and 4 contain WsaE. Lane 3, WsaE_N; lane 4, WsaE_M. The products were separated on silica TLC plates with chloroform/methanol/water (65:25:4) as solvent.