FIGURE 4.

Comparison of experimental and predicted MS spectra of Dol-16. Overlay of experimentally recorded MS spectrum of Dol-16 obtained from [1-¹³C]glucose feeding (red curve, cf. Fig. 3B) with two model theoretical spectra (hatched bars) calculated for Dol-16 synthesized exclusively by either the MEP or the MVA pathway. Location of the experimental data between the two theoretical distributions indicates mixed biosynthetic origin of dolichol. The signals of native dolichol (Fig. 3B) are overlaid with the theoretical spectrum (open bars) calculated for native Dol-16.