FIGURE 11.

Expression analysis of TPS6 and TPS11 in planta. A, transcript accumulation of tps6 and tps11 in roots and leaves of 14-day-old seedlings that were undamaged (ctr) or treated with S. littoralis for 12 h (dam). The bottom panel shows an ethidium bromide-stained agarose gel with total RNA as control for equal RNA loading. B, sesquiterpene hydrocarbons were extracted from leaves and roots of 14 day-old seedlings that were undamaged or treated with S. littoralis for 12 h. Compounds were identified and quantified as described under “Experimental Procedures.” Means and S.E. of six independent measurements are shown. Compounds marked with white bars were found in leaves and roots of both control and damaged plants. Compounds marked with black bars are products of the terpene synthases TPS6 and TPS11. Compounds marked with gray bars are products of TPS10. The compounds are as follows: α-copaene (1), (E)-β-caryophyllene (2), (E)-α-bergamotene (3), sesquisabinene A (4), (E)-β-farnesene (5), germacrene D (6), zingiberene (7), β-macrocarpene (8), β-bisabolene (9), δ-cadinene (10), and sesquiphellandrene (11).