Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Kinetic constants of various DAPDCs
| Km | kcat | kcat/Km | Relative activitya | |
|---|---|---|---|---|
| mm | min−1 | mm−1·min−1 | ||
| Wild type | 0.39 ± 0.02 | 939 ± 30 | 2441 ± 81 | 100 |
| I148L | 0.03 ± 0.01 | 23 ± 5 | 682 ± 125 | 28 |
| I148F | 0.11 ± 0.01 | 41 ± 5 | 378 ± 63 | 15 |
| I148A | 0.35 ± 0.04 | 20 ± 1 | 57 ± 5 | 2 |
| I148K | 0.36 ± 0.01 | 17 ± 1 | 47 ± 5 | 2 |
| I148D | 0.72 ± 0.03 | 4 ± 1 | 5 ± 1 | 0.2 |
| I148G | 0.36 ± 0.04 | 4 ± 1 | 12 ± 2 | 0.5 |
| EcDAPDC | 1.07 ± 0.13 | 1214 ± 211 | 1133 ± 135 | 46 |
The relative activities are calculated based on kcat/Km values.
