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Association of hXTP3-B with the membrane-anchored ubiquitin ligase complex. A, co-immunoprecipitation of hXTP3-B-long and -short with SEL1L. Cells mock-transfected or transfected with HA-tagged hXTP3-B or mouse EDEM1 (mEDEM1) were metabolically labeled for 16 h prior to extraction with 3% digitonin. non-imm, nonimmune rabbit serum. B, comparison of complex formation by hXTP3-B-long and HRD1 in lysis buffer containing 3% digitonin or 1% Nonidet P-40. Cells were labeled for 16 h.
