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PIM1 expression following docetaxel treatment is dependent on STAT3. A, immunoblots of RWPE-2 cells treated with 100 nm docetaxel for the indicated time. B, RWPE-2 cells were transfected with STAT3 mutant control oligonucleotides or STAT3 decoy oligonucleotides and incubated for 16 h. Cells were then treated with 100 nm docetaxel for 6 h, lysed, and analyzed by immunoblotting for PIM1, phospho-STAT3, and total STAT3.
