Figure 6.

Inactivation of the Hippo pathway contributes to anoikis evasion and correlates with cancer metastasis. (A) Lats1/2 knockdown impairs anoikis in RWPE cells. RWPE cells were transfected with control siRNA or siRNAs targeting Lats1 and Lats2. Cells were then cultured on tissue culture plates (Attach) or ultralow attachment plates in suspension. After 72 h, cells were collected and stained with PE Annexin V and analyzed by FACS. Experiments were done in duplicate. (B) YAP/TAZ knockdown restores anoikis in ACHN cells. Experiments were similar to that in A except that siRNAs against YAP and TAZ as well as ACHN cells were used. Experiments were done in duplicate. (C) Lats1/2 down-regulation correlates with prostate cancer metastasis. Box plots showing the expression value of Lats1 and Lats2 in benign adjacent, localized (PCa), and metastatic (Met) prostate tumors. P-value measures the statistical difference between metastatic prostate cancer when compared with benign tissue and localized prostate cancer. (D) A model of the Hippo pathway mediating the cell attachment signal to anoikis and cancer metastasis.