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. 2011 Dec 22;4:54. doi: 10.1186/1756-8722-4-54

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Copyright ©2011 Xu et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Expression of SALL4 and CD133 in IH tissues. (A) Immunohistochemistry staining of proliferative (1,3) and involuting (2,4) IH tissue sections with anti-SALL4 and CD133 antibodies. Magnification: 100x, inset 200x. (B) 1, Phase contrast image of IHs tumor spheres forming on Petri dishes with surrounding mature endothelial cells and/or fibroblasts. This was observed after 3 days of culture in serum-free media. Magnification: 100 ×. 2, the tumor spheres continue to grow under serum-free conditions while mature cells such as endothelial cells and fibroblasts do not. Magnification: 100x. 3, phase contrast image of high density tumor spheres in culture. Magnification: 100 ×. 4, comparison of IH tumor sphere (left) and induced pluripotent stem cell clone (right). Magnification: 100 ×.