Figure 5.

Gleevec treatment of K562 (BCR-ABL positive) cells induces translocation of telomerase out of the nucleoli. K562, HL60 and Jurkat cells were infected with GFP-hTERT. Representative immunofluorescence images of (a) K562 GFP-hTERT non-treated cells; (b) K562 GFP-hTERT treated cells with 1 μM Gleevec for 16 h; (c) HL60 GFP-hTERT non-treated cells; (d) HL60 GFP-hTERT treated cells with 1 μM Gleevec for 16 h; (e) Jurkat GFP-hTERT non-treated cells; and (f) Jurkat GFP-hTERT treated cells with 1 μM Gleevec for 16 h. (g) Representative enlarged image of GFP-hTERT infected K562 cells displaying colocalization of GFP-hTERT with fibrillarin. Blue: DAPI; Green: GFP-hTERT; Red: Fibrillarin. All images were taken using Olympus Fluoview FV1000. Bar, 30 μm