Table 1.

Three mouse breeding groups used in the study and the data summary.

Row number	Genotype of mice	# of mice	% of predicted genotype in mouse pups	% of actual genotype in mouse pups	ABR test results for hearing
Group1: Breeding of BACCx30;Gjb2loxp/WT;Foxg1Cre/+ mice and Gjb2loxp/loxP mice for testing overexpressing Cx30 to rescue hearing in cCx26 null mice. Following genotypes presented in rows 1–6 are possible.
1	BACCx30;Gjb2loxp/loxp;Foxg1cre/+	34	12.5%	15.2%	All were deaf
2	BACCx30; Gjb2loxp/WT;Foxg1cre/+	21	12.5%	9.4%	All showed normal hearing
3	BACCx30;Gjb2loxp/WT and BACCx30;Gjb2loxp/loxp	76	25%	34.1%	All showed normal hearing
4	Gjb2loxp/loxp;Foxg1cre/+	26	12.5%	11.6%	All were deaf
5	Gjb2loxp/WT;Foxg1cre/+	27	12.5%	12.3%	All showed normal hearing
6	Gjb2loxp/loxp and Gjb2loxp/WT	39	25%	17.5%	All showed normal hearing
	Total numbers of mice tested	223
Group2: Breeding of BACCx30;Gjb6+/− mice and Gjb6−/− mice for testing overexpressing Cx30 to rescue hearing in Cx30 null mice. Following genotypes presented in rows 7–9 are possible.
7	BACCx30;Gjb6−/− or BACCx30;Gjb6+/−	79	50%	54.1%	All showed normal hearing
8	Gjb6−/−	37	25%	25.3%	All were deaf
9	Gjb6+/−	30	25%	20.5%	All showed normal hearing
	Total numbers of mice tested	146
Group3: Breeding of BACCx26;Gjb2loxp/WT;Foxg1Cre/+ mice and Gjb2loxp/loxP mice for testing overexpressing Cx26 to rescue hearing in cCx26 null mice. Following genotypes presented in rows 10–14 are possible.
10	BACCx26;Gjb2loxp/loxp;Foxg1cre/+ or BACCx26;Gjb2loxp/WT;Foxg1cre/+	40	25%	23.8%	All showed normal hearing
11	BACCx26; Gjb2loxp/loxp and BACCx26; Gjb2loxp/WT	40	25%	23.8%	All showed normal hearing
12	Gjb2loxp/loxp; Foxg1cre/+	25	12.5%	14.9%	All were deaf
13	Gjb2loxp/WT;Foxg1cre/+	20	12.5%	11.9%	All showed normal hearing
14	Gjb2loxp/WT and Gjb2loxp/loxp	43	25%	25.6%	All showed normal hearing
	Total numbers of mice tested	168