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. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: Biomaterials. 2011 Dec 15;33(7):2041–2049. doi: 10.1016/j.biomaterials.2011.11.070

Figure 1. Schematic of microwell culture/EB differentiation process.

Figure 1

hESCs were cultured in 3-D microwells or on 2-D substrates for 6 days. At day 6 the cells were analyzed for E-cadherin expression, β-catenin localization, and Wnt target gene expression. For differentiation studies, on day 6 aggregates were enzymatically removed from either 3-D microwells or 2-D substrates and placed in suspension in medium containing serum. During the 5 day suspension period, Wnt reporter activity was assessed via assays for luciferase and GFP expression to quantify differences in Wnt signaling.