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. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: Biomaterials. 2011 Dec 15;33(7):2041–2049. doi: 10.1016/j.biomaterials.2011.11.070

Figure 6. Downregulation of canonical Wnt-responsive genes in microwell-cultured hESCs.

Figure 6

qPCR analysis of Wnt target gene expression in three microwell sizes (100×100×120, 300×300×120, and 500×500×120 µm) at day 6. Fold difference is relative to 2-D control (Matrigel-coated TCPS plates). Wnt downstream genes were downregulated in all microwells as compared to the 2-D control (* indicates P<0.05, ** indicates P<0.005, *** indicates P<0.0005 compared to same-day 2-D control), but there was no statistically significant difference between different microwell sizes.