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. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: Biomaterials. 2011 Dec 15;33(7):2041–2049. doi: 10.1016/j.biomaterials.2011.11.070

Figure 7. EBs from microwells exhibited higher Wnt activity following 1 day in suspension.

Figure 7

H9-7TGFP cells were cultured for 6 days in microwells or on 2-D Matrigel-coated TCPS plates. On day 6, aggregates were removed from the substrates and placed in suspension to form EBs. The EBs were collected at day 0 and day 1 and a luciferase assay was performed. Cells cultured in microwells exhibited less canonical Wnt signaling activity at Day 0, but after 24 hours in suspension the EBs from hESCs cultured in all three microwell sizes showed more luciferase expression than EBs from cells cultured on 2-D Matrigel-coated TCPS. Cells that were transfected using a scrambled sequence did not exhibit detectable luciferase activity (* indicates P<0.05 compared to same-day 2-D control).