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. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: Biomaterials. 2011 Dec 15;33(7):2041–2049. doi: 10.1016/j.biomaterials.2011.11.070

Figure 8. Microwell EBs showed earlier onset and higher levels of Wnt activity.

Figure 8

Flow cytometry for percentage GFP-positive cells using the H9-7TGFP Wnt reporter line. EBs from hESCs cultured on 2-D Matrigel-coated TCPS controls and in 300×300×120 µm microwells were analyzed at days 1–5 of suspension culture (A). Microwell-derived EBs contained a higher percentage of GFP-positive cells at each time point, and GFP expression peaked at Day 4. Comparison of percentage of GFP-positive cells at Day 4 in EBs from hESCs cultured on 2-D Matrigel-coated TCPS and hESCs cultured in 3 microwell sizes (100×100×120, 300×300×120, and 500×500×120 µm) (B) revealed that all microwell-derived EBs contained more GFP-positive cells than control EBs, with EBs from the 300×300×120 µm size exhibiting the highest percentage (ANOVA test, P<0.0005) of GFP-positive cells (* indicates P<0.05, ** indicates P<0.005, *** indicates P<0.0005 compared to same-day 2-D control).