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IGF-1R:E-cadherin complex. Co-immunoprecipitation of hTCEpi whole cell lysates using two separate IGF-1R antibodies confirmed an association between IGF-1Rβ and E-cadherin. (A) IGF-1Rβ was immunoprecipitated using a rabbit polyclonal antibody (Cell Signaling). (B) IGF-1Rβ clone C-20 was immunoprecipitated using a rabbit polyclonal antibody (Santa Cruz). Blotting for IGF-1Rβ was used to confirm successful immunoprecipitation. An irrelevant IgG was used as a control.
