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. Author manuscript; available in PMC: 2013 Jan 6.
Published in final edited form as: Biochem Biophys Res Commun. 2011 Dec 1;417(1):346–351. doi: 10.1016/j.bbrc.2011.11.113

Figure 1. Effect of HNE toxicity in RPE cells.

Figure 1

(A) RPE cells were treated with 0–70 μM of HNE for 12 h and assayed for cytotoxicity by MTT and LDH assay. (B) RPE cells were transiently transfected with empty p-Target vector (VT) and p-Target vector containing the ORF of hGSTA4 sequence (hGSTA4-Tr). VT and hGSTA4-Tr cells were treated with 0–70 μM of HNE for 12 h and assayed for cytotoxicity by MTT assay. The plots show the percent cell survival (mean ± SD, n = 4) at different concentrations of HNE. (C) Over-expression of hGSTA4-4 in transfected cells was confirmed by western blot analysis.