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. Author manuscript; available in PMC: 2013 Jan 6.
Published in final edited form as: Biochem Biophys Res Commun. 2011 Dec 1;417(1):346–351. doi: 10.1016/j.bbrc.2011.11.113

Figure 2. Effect of HNE on VEGF secretion and VEGFR-2 expression in RPE cells.

Figure 2

(A) Effect of HNE on VEGF secretion: RPE cells were transiently transfected with empty p-Target vector (VT) and p-Target vector containing the ORF of hGSTA4 sequence (hGSTA4-Tr). VT and hGSTA4-Tr cells were treated with 0–20 μM of HNE for 12 h and assayed for VEGF secretion by ELISA as mentioned in Method Section. The data represent the mean ± SD (n =3). (B) Effect of 4-HNE on VEGFR-2 expression: RPE cells were treated with 0–20 μM HNE. Cell extracts prepared as described in methods section containing 70 μg protein were subjected to Western blot analysis. Western blot analysis was performed with total cell lysates using indicated antibodies. β-actin was used to control for loading differences.