. Author manuscript; available in PMC: 2013 Jan 1.

Published in final edited form as: Eur J Med Chem. 2011 Nov 4;47C:167–174. doi: 10.1016/j.ejmech.2011.10.039

Table 4.

Inhibition of the growth of various solid tumor cell lines

Cell line	IC₅₀ of 6-substituted purine nucleoside analog (µM)
	12	15	16
SNB7 (CNS)	33	31	10
DLD-1 (colon)	170	6	0.9
NCI-H23 (lung)	1	1.2	0.6
ZR-75-1 (mammary)	23	1.2	0.3
LOXIMVI (melanoma)	>200	6.2	0.3
PC-3 (prostate)	67	1.2	0.9
CAKI-1 (renal)	>200	31	30

The above cells were plated in 96-well microtiter plates and incubated with various concentrations of compound number 12, 15, or 16 at 37 °C. Cell viability was measured after 72 hours of continuous incubation with compound using the sulforhodamine B assay (absorbance read at 570 nm), and the concentration of compound that inhibited cell growth by 50% was determined. The results shown are the result of one experiment.