Defective expression of FLR1 in swi2Δ, pdr1Δ, pdr3Δ, and yrr1Δ cells is partially suppressed by sin4Δ mutation. Indicated trains were grown in YPD medium at 30°C to an A600 of 1.0. Benomyl was added to a final concentration of 5 µg/ml and samples were collected after 0, 1, and 3 h. Total RNA was isolated and assayed for ACT1 and FLR1 transcripts by real-time RT-PCR. The results were normalized to ACT1 RNA and expressed relative to the value for the WT strain at 0 h. The experiment was repeated three times, and the results are shown as means ± SD.