Figure 5.

Model depicting the comparison of transcription in wild-type cells expressing intact mediator and transcription in sin4Δ cells. In sin4Δ cells, we found the tail module anchored to the promoter of the FLR1 gene, while the head/middle module was also found in the coding region. Interestingly, intact mediator was found not only in the promoters but also in the coding regions of number of strongly transcribed genes.²⁴ We speculate that in wild-type cells, the mediator partitions between the promoter and coding region, depending on the architecture of the promoter and interactions of the mediator with the DNA-binding transcriptional factors (TF), RNA Pol II, and other components of the transcriptional machinery.