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. Author manuscript; available in PMC: 2013 Jan 30.
Published in final edited form as: J Control Release. 2011 Sep 22;157(2):249–259. doi: 10.1016/j.jconrel.2011.09.074

Figure 5.

Figure 5

Confocal fluorescence micrographs showing internalization of DNA by COS-7 cells treated with (A) lipoplexes of reduced BFDMA, (B) lipoplexes of oxidized BFDMA or (C) lipoplexes of oxidized BFDMA treated with AA. Fluorescence signals correspond to Lysotracker Red (endosome/lysosome stain; blue), WGA-Alexa Fluor 488 (membrane stain; green), Cy5 (labeled DNA; red), and Hoechst 34580 (nuclear stain; signal). Thick white arrows point to lipoplexes that appear to be associated with cell membranes, thin white arrows point to internalized lipoplexes located in endosomes and/or lysosomes, and white arrow heads point to lipoplexes that are not located in acidic endosomes or lysosomes.