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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: J Neurochem. 2012 Feb;120(4):528–540. doi: 10.1111/j.1471-4159.2011.07604.x

Fig. 1.

Fig. 1

Comparison of binding of HA-Cbln1 and HA-Cbln2 to Grid2 and β-neurexins. (A) HEK293T cells were transfected with Grid2 or empty vector (Mock). Cells were subsequently exposed to conditioned medium (Input) containing HA-Cbln1 or HA-Cbln2 for 4 h. After washing with medium, cells were lysed with sample buffer and subjected to SDS-PAGE and immunoblotted with anti- HA antiserum. HA-Cbln1 and HA-Cbln2 show elevated binding to Grid2 compared with mock transfection. (B) HEK293T cells were transfected with Nrxn1β, Nrxn1β(−S4), Nrxn2β, Nrxn3β or empty vector (Mock). Then cells were incubated with conditioned medium (Input) containing HA-Cbln1 or HA-Cbln2 for 4 h. After washing with medium, cells were lysed with sample buffer and subjected to SDS-PAGE and immunoblotted with anti- HA. Compared with mock transfection, both HA-Cbln1 and HA-Cbln2 bound to Nrxn1β, Nrxn2β and Nrxn3β, but not to Nrxn1β lacking the S4 splice variant sequence.