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. Author manuscript; available in PMC: 2013 Jan 20.
Published in final edited form as: FEBS Lett. 2011 Dec 22;586(2):132–136. doi: 10.1016/j.febslet.2011.12.020

Figure 2. YobL-CT and YxiD-CT are cytotoxic RNases.

Figure 2

The synthesis of CT toxins and ssrA(DAS)-tagged antitoxin proteins was induced in wild-type E. coli (clp+) and ΔclpX ΔclpA cells as described in Methods. Constructs expressing YobL-CT with the His562Ala mutation and YxiD-CT with the His548Ala mutation were also expressed in wild-type E. coli (clp+). Cells were collected at the indicated times after induction and RNA isolated for gel analysis. The gel migration positions of rRNAs and tRNAs are indicated.