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. Author manuscript; available in PMC: 2013 Jan 6.
Published in final edited form as: Biochem Biophys Res Commun. 2011 Dec 7;417(1):546–551. doi: 10.1016/j.bbrc.2011.11.160

Fig. 2.

Fig. 2

UV-induced miR-22 upregulation is ATM-dependent. (A) HaCat and HEK293T cells were treated with UV (20J/m2), Cells were harvested at indicated timepoints and total cell extracts were subjected to Western blot using antibodies as indicated. (B) HEK293T cells were transfected with Luciferase miR-22 reporter. Cells were left untreated or treated with UV in the presence or absence of Ku55933 (10 µM). Luciferase activity was quantified at 6 h after UV radiation. Data from three independent experiments were pooled and shown as mean ± SD. **: p<0.01. (C) ATM wild type (+/+) and deficient (−/−) MEF cells were mock treated or treated with UV (20J/m2), miR-22 expression was examined with qPCR at 12h after UV treatment. **: p<0.01.