Multi-energy CT allows differentiation of iodine, barium and bone in mouse. Single-energy image demonstrates similar attenuation values of iodine, barium and bone, which cannot be distinguished on the basis of CT numbers at energies >23 keV (A). Multi-energy CT data enable differentiation of iodine in circulation (red colour coding), barium in lungs (yellow) and bone (white) on transverse (B) and volume-rendered CT images (C). Note iodine and barium both have high Z-numbers and are solely differentiated on the basis of k-edges being 4 keV apart. Left lower bronchus (LLB), left lower lobe (LLL), pulmonary trunk (PA), right atrium (RA), right lower lobe (RLL), right ventricle (RV)