Figure 3.

Effect of rapamycin combined with irradiation in vivo. Rapamycin was given for 5 days with IR administered either on the 6^th or 10^th day after the start of therapy. 3A) Intratumoral oxygen tension for all groups was measured just prior to the administration of IR. Treatment of tumors with 5 days of rapamycin just prior IR resulted in higher intratumoral oxygenation compared to control tumors (*p=0.0000009) when controlling for size differences. Oxygenation was also significantly higher in the group given 5 doses of rapamycin compared to IR only (^p=0.000005). 3B) Tumor volumes in the combination therapy groups are represented as the ratio of change in tumor volume from the start of therapy to fourteen days after the end of therapy. The cohort that received combination therapy with rapamycin plus IR on the 6^th day had significant tumor growth restriction compared to controls (*p=0.002). 3C) Tumors treated with 5 doses of rapamycin prior to IR had significantly slower tumor growth than tumors treated with IR first followed by 5 days of rapamycin (*p=0.001).