Table 2.
PgE2 metabolic pathway gene expression in control and AERD tissue.
| Gene | Control ΔCT 1 | AERD ΔCT | ΔΔCT (2ΔΔCT)2 |
|---|---|---|---|
| COX-1 | 10.5 ± 0.41 | 11.3 ± 2.1 | −0.8 (.57) |
| COX-2 | 6.0 ± 0.6 | 7.9 ± 2.8* | −1.9 (.27) |
| mPGES-1 | 4.0 ± 0.7 | 5.0 ± 0.5 | −1 (.5) |
| mPGES-2 | 2.8 ± 0.8 | 4.5 ± 0.4* | −1.7 (.31) |
| cPGES | −0.1 ± 0.6 | 0.0 ± 0.5 | +0.1 (1.07) |
*P < 0.05.
1Quantitiatve polymerase chain reaction data are presented as ΔCT, which is the difference in threshold cycle of expression of each gene compared to housekeeping gene (each cycle corresponds to ~1 log2 difference in mRNA concentration; a higher ΔCT represents less mRNA).
2ΔΔCT is the difference in ΔCT of gene expressed in control compared to AERD tissue.
2ΔΔCT is the relative expression of gene in AERD compared to control tissue.