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. Author manuscript; available in PMC: 2013 Jan 11.
Published in final edited form as: Structure. 2012 Jan 11;20(1):151–160. doi: 10.1016/j.str.2011.10.019

Figure 5.

Figure 5

Functional analysis of MRG15-Pf1 interactions in cells. Co-immunoprecipitation analysis of (A) various FLAG-tagged Pf1 mutants with wild-type HA-tagged MRG15 and (B) various HA-tagged MRG15 mutants with wild-type FLAG-tagged Pf1 conducted in HEK293T cells. (C) Transcriptional repression assays conducted in HEK293T cells with GAL4 DBD-fusions of wild-type and mutant MRG15 using a luciferase reporter and a thymidine kinase promoter harboring four tandem GAL4 DNA-binding sites.