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. Author manuscript; available in PMC: 2013 Jan 11.
Published in final edited form as: Structure. 2012 Jan 11;20(1):151–160. doi: 10.1016/j.str.2011.10.019

Table 2.

Equilibrium Dissociation Constants for Various MRG15 MRG-Pf1 Complexes

Reactants KD(μM)
MRG15 MRG + Pf1 (aa 114–258) 0.016 ± 0.004a
MRG15 MRG + Pf1 MBD 0.012 ±0.002
MRG15 MRG + Pf1 MBD F210A 2.6 ± 0.7
MRG15 MRG + Pf1 MBD L212A 0.035 ± 0.017a
MRG15 MRG + Pf1 MBD L213A 0.280 ± 0.063
MRG15 MRG + Pf1 MBD N221E 0.015 ± 0.001a
MRG15 MRG + Pf1 MBD F225A 19 ± 14
MRG15 MRG + Pf1 MBD L227A 0.370 ± 0.087
MRG15 MRG + Pf1 MBD P228A 0.085 ± 0.017
MRG15 MRG + Pf1 (aa 219–232) 119 ± 26b
Pf1 MBD + MRG15 MRG W172A 0.067 ± 0.013a
Pf1 MBD + MRG15 MRG R201Kc 0.023 ± 0.006
Pf1 MBD + MRG15 MRG V227A 0.194 ± 0.073
Pf1 MBD + MRG15 MRG M228A 0.075 ± 0.013
Pf1 MBD + MRG15 MRG Y235A – d
Pf1 MBD + MRG15 MRG V268A 0.184 ± 0.062
Pf1 MBD + MRG15 MRG M273A 0.096 ± 0.019
Pf1 MBD + MRG15 MRG L287A 0.407 ± 0.102
a

average values from two independent measurements; all others from at least three independent measurements

b

measured using NMR; all others measured using ITC

c

note that all studies with MRG15 MRG proteins were inadvertently conducted in a K201R mutant background

d

no detectable binding; hence could not be quantified