TABLE 3.
Effects of phytocannabinoids on recovery from open channel inactivation of Cav3 channels
Cells expressing recombinant Cav3 channels were voltage-clamped at −86 mV, stepped to −26 mV for 70 ms (Cav3.1 and Cav3.2) or 350 ms (Cav3.3) to inactivate the channels, and then retested with steps to −26 mV at 20, 40, 80, 160, 320, 640, 1280, and 2560 ms after the end of the initial depolarization. Recovery curves for Cav3.1 in the presence of THC and CBD are illustrated in Fig. 6. *, p < 0.05; **, p < 0.001 from control.
|
Drug |
CaV |
Recovery from inactivation |
|
|---|---|---|---|
| Control | In drug | ||
| ms | |||
| 1 μm THC | 3.1 | 63 ± 3 | 121 ± 10** |
| 1 μm THC | 3.2 | 224 ± 10 | 430 ± 7** |
| 3 μm THC | 3.3 | 211 ± 5 | 410 ± 20** |
| 1 μm CBD | 3.1 | 69 ± 2 | 167 ± 20** |
| 1 μm CBD | 3.2 | 230 ± 10 | 384 ± 58** |
| 3 μm CBD | 3.3 | 184 ± 4 | 436 ± 27** |