FIGURE 6.

Effects of USP11 on subsequent HPV-16E7-mediated protein activation and cell growth activation. A, the USP11 si-RNA leads to reduced HPV-16E7 and increased Rb in CaSki cells. 300-g cell extracts from CaSki (si) and CaSki (si-USP11) cell lines were subjected to Western blot analysis using α-USP11 or α-HPV-16E7 to detect the levels of endogenous HPV-16E7 and USP11 expression, respectively. Tubulin protein was assayed to monitor equal loading between individual lanes. Quantitative measurements of signals are presented on the right. B, colony formation assay. CaSki (si) and CaSki (si-USP11) cells were subcultured and kept in the medium containing G418 (200 μg/ml), and surviving colonies were counted 2 weeks later. Relative cell viability is summarized from the five separate experiments and is presented on the right.