FIGURE 2.

Dominant negative TRAF6 does not block IL-1α-induced mRNA stabilization. HeLa tet-off cells were transfected with 2 μgofKCΔ3, 2 μgof 5× κB luciferase reporter, and 2 μg of either empty vector or a dominant negative (DN) TRAF6. A, luciferase activity was determined following treatment with IL-1α (10 ng/ml) for 8 h. Values represent the mean of duplicate samples. -Fold increase was determined by comparing the treated samples to the untreated (NT) in the same transfection group. B, dox (1 μg/ml) was added to each sample either alone (NT) or with IL-1α (10 ng/ml). Total RNA was isolated following treatment for the indicated times, and KC and GAPDH mRNA levels were determined by northern hybridization. Data are representative of at least three independent experiments.