FIGURE 6.

14-3-3 inhibits the recovery of TRESK current after the stimulation of M₁ muscarinic receptor. A, normalized currents of two groups of oocytes microinjected with 50 nl of GST-h14-3-3η (wild type, wt., black curve) or R57A,R61A mutant GST-h14-3-3η fusion protein (gray curve). The cells were coexpressing M₁ muscarinic receptor with human TRESK and R57A,R61A 14-3-3η (triple coexpression in both groups). Extracellular [K⁺] was increased from 2 to 80 mm, and the oocytes were stimulated with carbachol (Carb., 1 μm, as indicated by the horizontal black bar) 200–233 min after the microinjection of the proteins. B, normalized recovery was calculated from the same recordings as represented in panel A.