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. 2008 Jun 6;283(23):16004–16016. doi: 10.1074/jbc.M708525200

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Copyright © 2008, The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — Differential expression of XHpaL and XHpaS during development. A, RT-PCR products for XHpaL, XHpaS, and EF1α generated from whole embryos at different stages of development. RT-PCR of one representative experiment is shown. Densitometric quantification expressed as the ratio between xHpaL and xHpaS and the number of determinations (n) indicated above each bar is also shown. Error bars represent the S.D. ^*, p < 0.05; analysis of variance. B, Western blot analysis of extracts from whole embryos (150 μg) at different stages of development using the anti-human heparanase antibody (clone HP3/17).β-Actin detection was used as the equal loading control.