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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Dev Biol. 2011 Nov 18;362(1):11–23. doi: 10.1016/j.ydbio.2011.10.036

Figure 1. eomesafh105 mutant allele.

Figure 1

Schematic of the 661 amino acid full-length Eomesa protein with T-domain in blue. Location of the stop codon in allele fh105 marked by asterisk. (B) Images of heterozygous (top) and homozygous (bottom) eomesafh105 adult fish. (C) Control western blot for the anti-Eomesa antibody, lanes as indicated. (D)Western blot of sphere stage wild type and MZeomesa embryos, lanes as indicated. 15 embryo equivalents loaded per lane. (E) Whole-mount in situ hybridization for eomesa on sphere stage embryos of the indicated genotypes.