FIGURE 5.

Preferential recruitment of HP1 on MyoD target promoters in proliferating myoblasts. Chromatin fractions from either proliferating C2C12 myoblasts (black bars) or cultured 48 h in differentiating medium (gray bars) were immunoprecipitated using anti-HP1 antibodies (HP1) and analyzed by quantitative PCR. We quantified copy numbers of the p21 and MCK promoters regions harboring the MyoD-binding site. gapdh and 36B4 genes were used as negative controls to normalize our ChIP results. The results are the means of two independent experiments and six PCR measurements.