FIGURE 2.

Binding of CaM to the synthetic peptides for two CaMBDs. The CaM-binding activity of the CaMBDI peptide (A) corresponding to amino acids 445–469 and the CaMBDII peptide (B) corresponding to amino acids 669–692 in AtMKP1 was analyzed using a gel mobility shift assay. AtCaM2 (303 pmol) was incubated with increasing amounts of peptide (peptide/CaM molar ratios are indicated) in the presence of 0.1 mm CaCl₂ or 2 mm EGTA. Samples were separated by nondenaturing PAGE. The positions of free CaM and the peptide-CaM complex are indicated by arrows.