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Analysis of the binding affinity between CaMBDs and CaM. A, effect of synthetic peptides derived from CaMBDs on the activation of PDE by CaM. Peptides competed with PDE for binding to CaM. PDE activity was measured in the presence of varying concentrations of AtCaM2, either in the presence or absence of a fixed concentration (100 nm) of two synthetic CaMBD peptides. B, Km and Kd values for the CaMBD peptides of AtMKP1.
