FIGURE 5.
Split ubiquitin assay to analyze the interaction between CaM and AtMKP1 in vivo. Shown are serial dilutions of cells co-expressing Nub or the Nub-AtMKP1 (WT and CaMBD mutant) fusion protein together with CaM (AtCaM2-Cub-RUra3), and Cub or the AtMKP1-Cub-RUra3 (WT and CaMBD mutant) fusion protein together with CaM (Nub-AtCaM2), on plates lacking tryptophan and histidine (control), or additionally lacking uracil (-Ura) or containing 5-FOA (+FOA). All proteins were expressed from single-copy vectors.